Construct: shRNA TRCN0000075223
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_005110.1-2345s1c1
- DNA Barcode:
- CCAGAGGAGAAGGGAATCATT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GFPT2 (9945)
- Gene Description:
- glutamine-fructose-6-phosphate transaminase 2
- Transcript:
- RefSeq NM_005110.1 (NON-CURRENT)
- Match location:
- Position 2345 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000075223)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCAGAGGAGAAGGGAATCATT
- Hairpin Sequence:
-
5'-CCGG-CCAGAGGAGAAGGGAATCATT-CTCGAG-AATGATTCCCTTCTCCTCTGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCAGAGGAGAAGGGAATCATTCTCGAGAATGATTCCCTTCTCCTCTGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCAGAGGAGAAGGGAATCATTCTCGAGAATGATTCCCTTCTCCTCTGG-3'
Other clones with same target sequence:
-
TRCN0000300712
