Construct: shRNA TRCN0000075233

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_174936.2-2979s1c1
DNA Barcode:
GCCACCTTTACTCTGCTCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCSK9 (255738)
Gene Description:
proprotein convertase subtilisin/kexin type 9
Transcript:
RefSeq NM_174936.2 (NON-CURRENT)
Match location:
Position 2979 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075233)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACCTTTACTCTGCTCTAT
Hairpin Sequence:
5'-CCGG-GCCACCTTTACTCTGCTCTAT-CTCGAG-ATAGAGCAGAGTAAAGGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACCTTTACTCTGCTCTATCTCGAGATAGAGCAGAGTAAAGGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACCTTTACTCTGCTCTATCTCGAGATAGAGCAGAGTAAAGGTGGC-3'

Other clones with same target sequence:

(none)