Construct: shRNA TRCN0000075268

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014893.2-3495s1c1
DNA Barcode:
GCCAGCCTGAACTATATTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NLGN4Y (22829)
Gene Description:
neuroligin 4 Y-linked
Transcript:
RefSeq NM_014893.2 (NON-CURRENT)
Match location:
Position 3495 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075268)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGCCTGAACTATATTTAA
Hairpin Sequence:
5'-CCGG-GCCAGCCTGAACTATATTTAA-CTCGAG-TTAAATATAGTTCAGGCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGCCTGAACTATATTTAACTCGAGTTAAATATAGTTCAGGCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGCCTGAACTATATTTAACTCGAGTTAAATATAGTTCAGGCTGGC-3'

Other clones with same target sequence:

(none)