Construct: shRNA TRCN0000075286

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031213.2-1145s1c1
DNA Barcode:
CACCAAGAAGACCTACTGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABHD17A (81926)
Gene Description:
abhydrolase domain containing 17A
Transcript:
RefSeq NM_031213.2 (NON-CURRENT)
Match location:
Position 1145 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075286)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCAAGAAGACCTACTGCTT
Hairpin Sequence:
5'-CCGG-CACCAAGAAGACCTACTGCTT-CTCGAG-AAGCAGTAGGTCTTCTTGGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCAAGAAGACCTACTGCTTCTCGAGAAGCAGTAGGTCTTCTTGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCAAGAAGACCTACTGCTTCTCGAGAAGCAGTAGGTCTTCTTGGTG-3'

Other clones with same target sequence:

(none)