Construct: shRNA TRCN0000075349

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010906.1-681s1c1
DNA Barcode:
CCGGACAATCAGATAGTTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nfix (18032)
Gene Description:
nuclear factor I/X
Transcript:
RefSeq NM_010906.1 (NON-CURRENT)
Match location:
Position 681 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075349)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGACAATCAGATAGTTCAA
Hairpin Sequence:
5'-CCGG-CCGGACAATCAGATAGTTCAA-CTCGAG-TTGAACTATCTGATTGTCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGACAATCAGATAGTTCAACTCGAGTTGAACTATCTGATTGTCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGACAATCAGATAGTTCAACTCGAGTTGAACTATCTGATTGTCCGG-3'

Other clones with same target sequence:

(none)