Construct: shRNA TRCN0000075377

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013627.2-1319s1c1
DNA Barcode:
CCTGAAGCAAGAATACAGGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pax6 (18508)
Gene Description:
paired box 6
Transcript:
RefSeq NM_013627.2 (NON-CURRENT)
Match location:
Position 1319 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075377)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAAGCAAGAATACAGGTA
Hairpin Sequence:
5'-CCGG-CCTGAAGCAAGAATACAGGTA-CTCGAG-TACCTGTATTCTTGCTTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAAGCAAGAATACAGGTACTCGAGTACCTGTATTCTTGCTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAAGCAAGAATACAGGTACTCGAGTACCTGTATTCTTGCTTCAGG-3'

Other clones with same target sequence:

(none)