Construct: shRNA TRCN0000075410

Construct Description:

• Construct Type: shRNA
• Other Identifiers: NM_007496.1-2503s1c1
• DNA Barcode: CGCTGACGAAAGTGCCAATAA

Original Target:

• Taxon: Mus musculus (mouse)
• Gene: Zfhx3 (11906)
• Gene Description: zinc finger homeobox 3
• Transcript: RefSeq NM_007496.1 (NON-CURRENT)
• Match location: Position 2503 (CDS)

Vector Information:

• Vector Backbone: pLKO.1
• Pol II Cassette 1: PGK-PuroR
• Pol II Cassette 2: n/a
• Selection Marker: PuroR
• Visible Reporter: n/a
• Epitope Tag: n/a

Current transcripts matched by this shRNA:

	Taxon	Gene	Symbol	Description	Transcript	SDR Match %	Region	Start Pos.	Intrinsic Score	Adjusted Score
1	mouse	11906	Zfhx3	zinc finger homeobox 3	NM_007496.2	100%	CDS	2506	13.200	18.480
2	mouse	11906	Zfhx3	zinc finger homeobox 3	XM_006530585.3	100%	CDS	1878	13.200	18.480
3	human	463	ZFHX3	zinc finger homeobox 3	NM_006885.4	100%	CDS	2196	13.200	9.240
4	human	463	ZFHX3	zinc finger homeobox 3	XM_005255957.4	100%	CDS	2394	13.200	9.240
5	human	59344	ALOXE3	arachidonate lipoxygenase 3	NM_001165960.1	89%	3UTR	2885
6	human	59344	ALOXE3	arachidonate lipoxygenase 3	NM_021628.2	89%	3UTR	2721

Sequence Information

• Target Sequence: CGCTGACGAAAGTGCCAATAA
• Hairpin Sequence: 5'-CCGG-CGCTGACGAAAGTGCCAATAA-CTCGAG-TTATTGGCACTTTCGTCAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

• Forward sequence: 5'-CCGGCGCTGACGAAAGTGCCAATAACTCGAGTTATTGGCACTTTCGTCAGCGTTTTTG-3'
• Reverse sequence: 5'-AATTCAAAAACGCTGACGAAAGTGCCAATAACTCGAGTTATTGGCACTTTCGTCAGCG-3'

Other clones with same target sequence:

(none)