Construct: shRNA TRCN0000075473

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007960.1-2448s1c1
DNA Barcode:
CGGAATCTTCATTTAAGTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Etv1 (14009)
Gene Description:
ets variant 1
Transcript:
RefSeq NM_007960.1 (NON-CURRENT)
Match location:
Position 2448 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075473)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAATCTTCATTTAAGTGTT
Hairpin Sequence:
5'-CCGG-CGGAATCTTCATTTAAGTGTT-CTCGAG-AACACTTAAATGAAGATTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAATCTTCATTTAAGTGTTCTCGAGAACACTTAAATGAAGATTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAATCTTCATTTAAGTGTTCTCGAGAACACTTAAATGAAGATTCCG-3'

Other clones with same target sequence:

(none)