Construct: shRNA TRCN0000075534

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009560.1-1577s1c1
DNA Barcode:
GCCTTGTGGAACATAGGATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp60 (22718)
Gene Description:
zinc finger protein 60
Transcript:
RefSeq NM_009560.1 (NON-CURRENT)
Match location:
Position 1577 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075534)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTGTGGAACATAGGATTA
Hairpin Sequence:
5'-CCGG-GCCTTGTGGAACATAGGATTA-CTCGAG-TAATCCTATGTTCCACAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTGTGGAACATAGGATTACTCGAGTAATCCTATGTTCCACAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTGTGGAACATAGGATTACTCGAGTAATCCTATGTTCCACAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000301412