Construct: shRNA TRCN0000075587

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_486109.1-787s1c1
DNA Barcode:
GAGGAAGAAGAGGAGGATGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hmgb2 (97165)
Gene Description:
high mobility group box 2
Transcript:
RefSeq XM_486109.1 (NON-CURRENT)
Match location:
Position 787 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075587)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGAAGAAGAGGAGGATGAA
Hairpin Sequence:
5'-CCGG-GAGGAAGAAGAGGAGGATGAA-CTCGAG-TTCATCCTCCTCTTCTTCCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGAAGAAGAGGAGGATGAACTCGAGTTCATCCTCCTCTTCTTCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGAAGAAGAGGAGGATGAACTCGAGTTCATCCTCCTCTTCTTCCTC-3'

Other clones with same target sequence:

  1. TRCN0000301335
  2. TRCN0000173568