Construct: shRNA TRCN0000075603

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053075.2-740s1c1
DNA Barcode:
CCCGTCATCCTTGAAGATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rheb (19744)
Gene Description:
Ras homolog enriched in brain
Transcript:
RefSeq NM_053075.2 (NON-CURRENT)
Match location:
Position 740 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075603)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGTCATCCTTGAAGATAAA
Hairpin Sequence:
5'-CCGG-CCCGTCATCCTTGAAGATAAA-CTCGAG-TTTATCTTCAAGGATGACGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGTCATCCTTGAAGATAAACTCGAGTTTATCTTCAAGGATGACGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGTCATCCTTGAAGATAAACTCGAGTTTATCTTCAAGGATGACGGG-3'

Other clones with same target sequence:

(none)