Construct: shRNA TRCN0000075699

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173370.3-730s1c1
DNA Barcode:
GCAGATTACTTCGCCACGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cds1 (74596)
Gene Description:
CDP-diacylglycerol synthase 1
Transcript:
RefSeq NM_173370.3 (CURRENT)
Match location:
Position 730 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075699)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATTACTTCGCCACGTTT
Hairpin Sequence:
5'-CCGG-GCAGATTACTTCGCCACGTTT-CTCGAG-AAACGTGGCGAAGTAATCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATTACTTCGCCACGTTTCTCGAGAAACGTGGCGAAGTAATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATTACTTCGCCACGTTTCTCGAGAAACGTGGCGAAGTAATCTGC-3'

Other clones with same target sequence:

(none)