Construct: shRNA TRCN0000075734

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013930.2-1007s1c1
DNA Barcode:
GCCCTATACAACTTGTTTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aass (30956)
Gene Description:
aminoadipate-semialdehyde synthase
Transcript:
RefSeq NM_013930.2 (NON-CURRENT)
Match location:
Position 1007 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075734)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTATACAACTTGTTTAAT
Hairpin Sequence:
5'-CCGG-GCCCTATACAACTTGTTTAAT-CTCGAG-ATTAAACAAGTTGTATAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTATACAACTTGTTTAATCTCGAGATTAAACAAGTTGTATAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTATACAACTTGTTTAATCTCGAGATTAAACAAGTTGTATAGGGC-3'

Other clones with same target sequence:

  1. TRCN0000287709