Construct: shRNA TRCN0000075769

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175400.5-663s1c1
DNA Barcode:
CCGTATATGATGGGCAGGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sephs1 (109079)
Gene Description:
selenophosphate synthetase 1
Transcript:
RefSeq NM_175400.5 (NON-CURRENT)
Match location:
Position 663 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075769)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTATATGATGGGCAGGATA
Hairpin Sequence:
5'-CCGG-CCGTATATGATGGGCAGGATA-CTCGAG-TATCCTGCCCATCATATACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTATATGATGGGCAGGATACTCGAGTATCCTGCCCATCATATACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTATATGATGGGCAGGATACTCGAGTATCCTGCCCATCATATACGG-3'

Other clones with same target sequence:

(none)