Construct: shRNA TRCN0000075777

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007506.2-224s1c1
DNA Barcode:
CAGTGTCATTTCCCGGGACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp5g1 (11951)
Gene Description:
ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C1 (subunit 9)
Transcript:
RefSeq NM_007506.2 (NON-CURRENT)
Match location:
Position 224 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075777)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTGTCATTTCCCGGGACAT
Hairpin Sequence:
5'-CCGG-CAGTGTCATTTCCCGGGACAT-CTCGAG-ATGTCCCGGGAAATGACACTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTGTCATTTCCCGGGACATCTCGAGATGTCCCGGGAAATGACACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTGTCATTTCCCGGGACATCTCGAGATGTCCCGGGAAATGACACTG-3'

Other clones with same target sequence:

  1. TRCN0000318093