Construct: shRNA TRCN0000075830

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008256.2-1170s1c1
DNA Barcode:
CCTCTCCACAAATAATGGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hmgcs2 (15360)
Gene Description:
3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2
Transcript:
RefSeq NM_008256.2 (NON-CURRENT)
Match location:
Position 1170 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075830)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTCCACAAATAATGGGAA
Hairpin Sequence:
5'-CCGG-CCTCTCCACAAATAATGGGAA-CTCGAG-TTCCCATTATTTGTGGAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTCCACAAATAATGGGAACTCGAGTTCCCATTATTTGTGGAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTCCACAAATAATGGGAACTCGAGTTCCCATTATTTGTGGAGAGG-3'

Other clones with same target sequence:

(none)