Construct: shRNA TRCN0000075907

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008214.2-304s1c1
DNA Barcode:
CCTGTGTTTGAACTAAAGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hars (15115)
Gene Description:
histidyl-tRNA synthetase
Transcript:
RefSeq NM_008214.2 (NON-CURRENT)
Match location:
Position 304 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075907)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTGTTTGAACTAAAGGAA
Hairpin Sequence:
5'-CCGG-CCTGTGTTTGAACTAAAGGAA-CTCGAG-TTCCTTTAGTTCAAACACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTGTTTGAACTAAAGGAACTCGAGTTCCTTTAGTTCAAACACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTGTTTGAACTAAAGGAACTCGAGTTCCTTTAGTTCAAACACAGG-3'

Other clones with same target sequence:

  1. TRCN0000325939