Construct: shRNA TRCN0000075964

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146217.3-966s1c1
DNA Barcode:
CCTCGTGTTCATCCAGTATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aars (234734)
Gene Description:
alanyl-tRNA synthetase
Transcript:
RefSeq NM_146217.3 (NON-CURRENT)
Match location:
Position 966 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075964)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCGTGTTCATCCAGTATAA
Hairpin Sequence:
5'-CCGG-CCTCGTGTTCATCCAGTATAA-CTCGAG-TTATACTGGATGAACACGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCGTGTTCATCCAGTATAACTCGAGTTATACTGGATGAACACGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCGTGTTCATCCAGTATAACTCGAGTTATACTGGATGAACACGAGG-3'

Other clones with same target sequence:

  1. TRCN0000309809