Construct: shRNA TRCN0000075976

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_128656.4-1159s1c1
DNA Barcode:
TGAGAAGAACTCAGTCAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aars2 (224805)
Gene Description:
alanyl-tRNA synthetase 2, mitochondrial
Transcript:
RefSeq XM_128656.4 (NON-CURRENT)
Match location:
Position 1159 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000075976)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAGAAGAACTCAGTCAAGAT
Hairpin Sequence:
5'-CCGG-TGAGAAGAACTCAGTCAAGAT-CTCGAG-ATCTTGACTGAGTTCTTCTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAGAAGAACTCAGTCAAGATCTCGAGATCTTGACTGAGTTCTTCTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAGAAGAACTCAGTCAAGATCTCGAGATCTTGACTGAGTTCTTCTCA-3'

Other clones with same target sequence:

(none)