Construct: shRNA TRCN0000076045

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145942.2-1328s1c1
DNA Barcode:
GCAAGTTTATGTGACCTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hmgcs1 (208715)
Gene Description:
3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1
Transcript:
RefSeq NM_145942.2 (NON-CURRENT)
Match location:
Position 1328 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000076045)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGTTTATGTGACCTTAAA
Hairpin Sequence:
5'-CCGG-GCAAGTTTATGTGACCTTAAA-CTCGAG-TTTAAGGTCACATAAACTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGTTTATGTGACCTTAAACTCGAGTTTAAGGTCACATAAACTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGTTTATGTGACCTTAAACTCGAGTTTAAGGTCACATAAACTTGC-3'

Other clones with same target sequence:

(none)