Construct: shRNA TRCN0000076052

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019455.2-560s1c1
DNA Barcode:
GCCTGGCTTGTTGGATATCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hpgds (54486)
Gene Description:
hematopoietic prostaglandin D synthase
Transcript:
RefSeq NM_019455.2 (NON-CURRENT)
Match location:
Position 560 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000076052)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGGCTTGTTGGATATCTA
Hairpin Sequence:
5'-CCGG-GCCTGGCTTGTTGGATATCTA-CTCGAG-TAGATATCCAACAAGCCAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGGCTTGTTGGATATCTACTCGAGTAGATATCCAACAAGCCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGGCTTGTTGGATATCTACTCGAGTAGATATCCAACAAGCCAGGC-3'

Other clones with same target sequence:

  1. TRCN0000302801