Construct: shRNA TRCN0000076054

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_129894.4-2236s1c1
DNA Barcode:
CGTATGCAAACAGCTACCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Acsl3 (74205)
Gene Description:
acyl-CoA synthetase long-chain family member 3
Transcript:
RefSeq XM_129894.4 (NON-CURRENT)
Match location:
Position 2236 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000076054)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTATGCAAACAGCTACCATT
Hairpin Sequence:
5'-CCGG-CGTATGCAAACAGCTACCATT-CTCGAG-AATGGTAGCTGTTTGCATACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTATGCAAACAGCTACCATTCTCGAGAATGGTAGCTGTTTGCATACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTATGCAAACAGCTACCATTCTCGAGAATGGTAGCTGTTTGCATACG-3'

Other clones with same target sequence:

(none)