Construct: shRNA TRCN0000076060

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153168.1-1973s1c1
DNA Barcode:
GCCTCATTAAGGGACAGACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lars2 (102436)
Gene Description:
leucyl-tRNA synthetase, mitochondrial
Transcript:
RefSeq NM_153168.1 (NON-CURRENT)
Match location:
Position 1973 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000076060)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCATTAAGGGACAGACAT
Hairpin Sequence:
5'-CCGG-GCCTCATTAAGGGACAGACAT-CTCGAG-ATGTCTGTCCCTTAATGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCATTAAGGGACAGACATCTCGAGATGTCTGTCCCTTAATGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCATTAAGGGACAGACATCTCGAGATGTCTGTCCCTTAATGAGGC-3'

Other clones with same target sequence:

(none)