Construct: shRNA TRCN0000076067

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144823.1-400s1c1
DNA Barcode:
CTTACCCATTACTATGACGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Acsl6 (216739)
Gene Description:
acyl-CoA synthetase long-chain family member 6
Transcript:
RefSeq NM_144823.1 (NON-CURRENT)
Match location:
Position 400 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000076067)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTACCCATTACTATGACGAT
Hairpin Sequence:
5'-CCGG-CTTACCCATTACTATGACGAT-CTCGAG-ATCGTCATAGTAATGGGTAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTACCCATTACTATGACGATCTCGAGATCGTCATAGTAATGGGTAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTACCCATTACTATGACGATCTCGAGATCGTCATAGTAATGGGTAAG-3'

Other clones with same target sequence:

(none)