Construct: shRNA TRCN0000076073

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178053.2-1545s1c1
DNA Barcode:
GCTCTACCTAAGGTAACCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nags (217214)
Gene Description:
N-acetylglutamate synthase
Transcript:
RefSeq NM_178053.2 (NON-CURRENT)
Match location:
Position 1545 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000076073)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTACCTAAGGTAACCTTA
Hairpin Sequence:
5'-CCGG-GCTCTACCTAAGGTAACCTTA-CTCGAG-TAAGGTTACCTTAGGTAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTACCTAAGGTAACCTTACTCGAGTAAGGTTACCTTAGGTAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTACCTAAGGTAACCTTACTCGAGTAAGGTTACCTTAGGTAGAGC-3'

Other clones with same target sequence:

(none)