Construct: shRNA TRCN0000076135

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025983.2-236s1c1
DNA Barcode:
GAAAGTCTCGAAGAAGGAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp5e (67126)
Gene Description:
ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit
Transcript:
RefSeq NM_025983.2 (NON-CURRENT)
Match location:
Position 236 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000076135)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGTCTCGAAGAAGGAGTA
Hairpin Sequence:
5'-CCGG-GAAAGTCTCGAAGAAGGAGTA-CTCGAG-TACTCCTTCTTCGAGACTTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGTCTCGAAGAAGGAGTACTCGAGTACTCCTTCTTCGAGACTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGTCTCGAAGAAGGAGTACTCGAGTACTCCTTCTTCGAGACTTTC-3'

Other clones with same target sequence:

  1. TRCN0000334639