Construct: shRNA TRCN0000076174

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134469.2-953s1c1
DNA Barcode:
CGCCAGATCTTAGAGGAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fdps (110196)
Gene Description:
farnesyl diphosphate synthetase
Transcript:
RefSeq NM_134469.2 (NON-CURRENT)
Match location:
Position 953 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000076174)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCAGATCTTAGAGGAGAAT
Hairpin Sequence:
5'-CCGG-CGCCAGATCTTAGAGGAGAAT-CTCGAG-ATTCTCCTCTAAGATCTGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCAGATCTTAGAGGAGAATCTCGAGATTCTCCTCTAAGATCTGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCAGATCTTAGAGGAGAATCTCGAGATTCTCCTCTAAGATCTGGCG-3'

Other clones with same target sequence:

  1. TRCN0000325549