Construct: shRNA TRCN0000076255

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010236.1-421s1c1
DNA Barcode:
CGGATTCGAATCAACGGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fpgs (14287)
Gene Description:
folylpolyglutamyl synthetase
Transcript:
RefSeq NM_010236.1 (NON-CURRENT)
Match location:
Position 421 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000076255)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGATTCGAATCAACGGGAAA
Hairpin Sequence:
5'-CCGG-CGGATTCGAATCAACGGGAAA-CTCGAG-TTTCCCGTTGATTCGAATCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGATTCGAATCAACGGGAAACTCGAGTTTCCCGTTGATTCGAATCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGATTCGAATCAACGGGAAACTCGAGTTTCCCGTTGATTCGAATCCG-3'

Other clones with same target sequence:

(none)