Construct: shRNA TRCN0000076265

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008217.2-1602s1c1
DNA Barcode:
CGTGAACTTCATTGGCCTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Has3 (15118)
Gene Description:
hyaluronan synthase 3
Transcript:
RefSeq NM_008217.2 (NON-CURRENT)
Match location:
Position 1602 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000076265)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGAACTTCATTGGCCTAAT
Hairpin Sequence:
5'-CCGG-CGTGAACTTCATTGGCCTAAT-CTCGAG-ATTAGGCCAATGAAGTTCACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGAACTTCATTGGCCTAATCTCGAGATTAGGCCAATGAAGTTCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGAACTTCATTGGCCTAATCTCGAGATTAGGCCAATGAAGTTCACG-3'

Other clones with same target sequence:

(none)