Construct: shRNA TRCN0000076323
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_021486.2-1952s1c1
- DNA Barcode:
- CAGAGAAATCCTATCTTGAAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Bco1 (63857)
- Gene Description:
- beta-carotene oxygenase 1
- Transcript:
- RefSeq NM_021486.2 (NON-CURRENT)
- Match location:
- Position 1952 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000076323)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CAGAGAAATCCTATCTTGAAA
- Hairpin Sequence:
-
5'-CCGG-CAGAGAAATCCTATCTTGAAA-CTCGAG-TTTCAAGATAGGATTTCTCTG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCAGAGAAATCCTATCTTGAAACTCGAGTTTCAAGATAGGATTTCTCTGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACAGAGAAATCCTATCTTGAAACTCGAGTTTCAAGATAGGATTTCTCTG-3'
Other clones with same target sequence:
(none)
