Construct: shRNA TRCN0000076364
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_010231.2-1277s1c1
- DNA Barcode:
- CCCATTGACATCATCGTCTTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Fmo1 (14261)
- Gene Description:
- flavin containing monooxygenase 1
- Transcript:
- RefSeq NM_010231.2 (NON-CURRENT)
- Match location:
- Position 1277 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000076364)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCCATTGACATCATCGTCTTT
- Hairpin Sequence:
-
5'-CCGG-CCCATTGACATCATCGTCTTT-CTCGAG-AAAGACGATGATGTCAATGGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCCATTGACATCATCGTCTTTCTCGAGAAAGACGATGATGTCAATGGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCCATTGACATCATCGTCTTTCTCGAGAAAGACGATGATGTCAATGGG-3'
Other clones with same target sequence:
(none)
