Construct: shRNA TRCN0000076367

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010231.2-1328s1c1
DNA Barcode:
CTCGATGAATCCGTAGTGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fmo1 (14261)
Gene Description:
flavin containing monooxygenase 1
Transcript:
RefSeq NM_010231.2 (NON-CURRENT)
Match location:
Position 1328 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000076367)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCGATGAATCCGTAGTGAAA
Hairpin Sequence:
5'-CCGG-CTCGATGAATCCGTAGTGAAA-CTCGAG-TTTCACTACGGATTCATCGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCGATGAATCCGTAGTGAAACTCGAGTTTCACTACGGATTCATCGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCGATGAATCCGTAGTGAAACTCGAGTTTCACTACGGATTCATCGAG-3'

Other clones with same target sequence:

(none)