Construct: shRNA TRCN0000076396

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011739.1-220s1c1
DNA Barcode:
CATCTCGAGCATTGAGCAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ywhaq (22630)
Gene Description:
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta
Transcript:
RefSeq NM_011739.1 (NON-CURRENT)
Match location:
Position 220 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000076396)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCTCGAGCATTGAGCAGAA
Hairpin Sequence:
5'-CCGG-CATCTCGAGCATTGAGCAGAA-CTCGAG-TTCTGCTCAATGCTCGAGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCTCGAGCATTGAGCAGAACTCGAGTTCTGCTCAATGCTCGAGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCTCGAGCATTGAGCAGAACTCGAGTTCTGCTCAATGCTCGAGATG-3'

Other clones with same target sequence:

  1. TRCN0000312369