Construct: shRNA TRCN0000076431

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133217.1-891s1c1
DNA Barcode:
CCCAGGAATGTACTACAGCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bco2 (170752)
Gene Description:
beta-carotene oxygenase 2
Transcript:
RefSeq NM_133217.1 (NON-CURRENT)
Match location:
Position 891 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000076431)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGGAATGTACTACAGCAT
Hairpin Sequence:
5'-CCGG-CCCAGGAATGTACTACAGCAT-CTCGAG-ATGCTGTAGTACATTCCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGGAATGTACTACAGCATCTCGAGATGCTGTAGTACATTCCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGGAATGTACTACAGCATCTCGAGATGCTGTAGTACATTCCTGGG-3'

Other clones with same target sequence:

(none)