Construct: shRNA TRCN0000076458

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133809.1-1801s1c1
DNA Barcode:
GCCTAGTGCTACTTCCTCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kmo (98256)
Gene Description:
kynurenine 3-monooxygenase (kynurenine 3-hydroxylase)
Transcript:
RefSeq NM_133809.1 (CURRENT)
Match location:
Position 1801 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000076458)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTAGTGCTACTTCCTCTTA
Hairpin Sequence:
5'-CCGG-GCCTAGTGCTACTTCCTCTTA-CTCGAG-TAAGAGGAAGTAGCACTAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTAGTGCTACTTCCTCTTACTCGAGTAAGAGGAAGTAGCACTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTAGTGCTACTTCCTCTTACTCGAGTAAGAGGAAGTAGCACTAGGC-3'

Other clones with same target sequence:

(none)