Construct: shRNA TRCN0000076470
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_011031.1-897s1c1
- DNA Barcode:
- GCGGTACTTTGAACGGTTGTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- P4ha2 (18452)
- Gene Description:
- procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha II polypeptide
- Transcript:
- RefSeq NM_011031.1 (NON-CURRENT)
- Match location:
- Position 897 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000076470)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCGGTACTTTGAACGGTTGTT
- Hairpin Sequence:
-
5'-CCGG-GCGGTACTTTGAACGGTTGTT-CTCGAG-AACAACCGTTCAAAGTACCGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCGGTACTTTGAACGGTTGTTCTCGAGAACAACCGTTCAAAGTACCGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCGGTACTTTGAACGGTTGTTCTCGAGAACAACCGTTCAAAGTACCGC-3'
Other clones with same target sequence:
-
TRCN0000326557
