Construct: shRNA TRCN0000076485

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009945.2-287s1c1
DNA Barcode:
CTGTTAGCTATGGCTGCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cox7a2 (12866)
Gene Description:
cytochrome c oxidase subunit VIIa 2
Transcript:
RefSeq NM_009945.2 (NON-CURRENT)
Match location:
Position 287 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000076485)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTTAGCTATGGCTGCATTT
Hairpin Sequence:
5'-CCGG-CTGTTAGCTATGGCTGCATTT-CTCGAG-AAATGCAGCCATAGCTAACAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTTAGCTATGGCTGCATTTCTCGAGAAATGCAGCCATAGCTAACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTTAGCTATGGCTGCATTTCTCGAGAAATGCAGCCATAGCTAACAG-3'

Other clones with same target sequence:

(none)