Construct: shRNA TRCN0000076487

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009945.2-304s1c1
DNA Barcode:
ATTTCCCAAGAAGCAGAACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cox7a2 (12866)
Gene Description:
cytochrome c oxidase subunit VIIa 2
Transcript:
RefSeq NM_009945.2 (NON-CURRENT)
Match location:
Position 304 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000076487)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTTCCCAAGAAGCAGAACTA
Hairpin Sequence:
5'-CCGG-ATTTCCCAAGAAGCAGAACTA-CTCGAG-TAGTTCTGCTTCTTGGGAAAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTTCCCAAGAAGCAGAACTACTCGAGTAGTTCTGCTTCTTGGGAAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTTCCCAAGAAGCAGAACTACTCGAGTAGTTCTGCTTCTTGGGAAAT-3'

Other clones with same target sequence:

  1. TRCN0000326625