Construct: shRNA TRCN0000076498

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025628.1-336s1c1
DNA Barcode:
CGCATAGCTGAAGGCACATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cox6b1 (110323)
Gene Description:
cytochrome c oxidase, subunit VIb polypeptide 1
Transcript:
RefSeq NM_025628.1 (NON-CURRENT)
Match location:
Position 336 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000076498)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCATAGCTGAAGGCACATTT
Hairpin Sequence:
5'-CCGG-CGCATAGCTGAAGGCACATTT-CTCGAG-AAATGTGCCTTCAGCTATGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCATAGCTGAAGGCACATTTCTCGAGAAATGTGCCTTCAGCTATGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCATAGCTGAAGGCACATTTCTCGAGAAATGTGCCTTCAGCTATGCG-3'

Other clones with same target sequence:

(none)