Construct: shRNA TRCN0000076569

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009676.1-475s1c1
DNA Barcode:
GCCCATAATTGATGCCTGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aox1 (11761)
Gene Description:
aldehyde oxidase 1
Transcript:
RefSeq NM_009676.1 (NON-CURRENT)
Match location:
Position 475 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000076569)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCATAATTGATGCCTGCAA
Hairpin Sequence:
5'-CCGG-GCCCATAATTGATGCCTGCAA-CTCGAG-TTGCAGGCATCAATTATGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCATAATTGATGCCTGCAACTCGAGTTGCAGGCATCAATTATGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCATAATTGATGCCTGCAACTCGAGTTGCAGGCATCAATTATGGGC-3'

Other clones with same target sequence:

(none)