Construct: shRNA TRCN0000076615

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172262.1-1065s1c1
DNA Barcode:
CCTGGCTTTGAGAAACCTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kdm1b (218214)
Gene Description:
lysine (K)-specific demethylase 1B
Transcript:
RefSeq NM_172262.1 (NON-CURRENT)
Match location:
Position 1065 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000076615)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGCTTTGAGAAACCTCAT
Hairpin Sequence:
5'-CCGG-CCTGGCTTTGAGAAACCTCAT-CTCGAG-ATGAGGTTTCTCAAAGCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGCTTTGAGAAACCTCATCTCGAGATGAGGTTTCTCAAAGCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGCTTTGAGAAACCTCATCTCGAGATGAGGTTTCTCAAAGCCAGG-3'

Other clones with same target sequence:

(none)