Construct: shRNA TRCN0000076706

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053083.1-327s1c1
DNA Barcode:
CCTAGATCAGTGTGGCTCTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Loxl4 (67573)
Gene Description:
lysyl oxidase-like 4
Transcript:
RefSeq NM_053083.1 (NON-CURRENT)
Match location:
Position 327 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000076706)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAGATCAGTGTGGCTCTAA
Hairpin Sequence:
5'-CCGG-CCTAGATCAGTGTGGCTCTAA-CTCGAG-TTAGAGCCACACTGATCTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAGATCAGTGTGGCTCTAACTCGAGTTAGAGCCACACTGATCTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAGATCAGTGTGGCTCTAACTCGAGTTAGAGCCACACTGATCTAGG-3'

Other clones with same target sequence:

(none)