Construct: shRNA TRCN0000076857
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_008866.2-499s1c1
- DNA Barcode:
- GCTAATCGAGATATTTCCGTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Lypla1 (18777)
- Gene Description:
- lysophospholipase 1
- Transcript:
- RefSeq NM_008866.2 (CURRENT)
- Match location:
- Position 499 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000076857)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTAATCGAGATATTTCCGTT
- Hairpin Sequence:
-
5'-CCGG-GCTAATCGAGATATTTCCGTT-CTCGAG-AACGGAAATATCTCGATTAGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTAATCGAGATATTTCCGTTCTCGAGAACGGAAATATCTCGATTAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTAATCGAGATATTTCCGTTCTCGAGAACGGAAATATCTCGATTAGC-3'
Other clones with same target sequence:
-
TRCN0000324616
