Construct: shRNA TRCN0000076913

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008874.2-4040s1c1
DNA Barcode:
CCCATAACATTCACCTAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plcb3 (18797)
Gene Description:
phospholipase C, beta 3
Transcript:
RefSeq NM_008874.2 (NON-CURRENT)
Match location:
Position 4040 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000076913)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATAACATTCACCTAAGAT
Hairpin Sequence:
5'-CCGG-CCCATAACATTCACCTAAGAT-CTCGAG-ATCTTAGGTGAATGTTATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATAACATTCACCTAAGATCTCGAGATCTTAGGTGAATGTTATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATAACATTCACCTAAGATCTCGAGATCTTAGGTGAATGTTATGGG-3'

Other clones with same target sequence:

  1. TRCN0000332215