Construct: shRNA TRCN0000076915

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008874.2-2640s1c1
DNA Barcode:
GCTTCTGACTACATCCCAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plcb3 (18797)
Gene Description:
phospholipase C, beta 3
Transcript:
RefSeq NM_008874.2 (NON-CURRENT)
Match location:
Position 2640 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000076915)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCTGACTACATCCCAGAT
Hairpin Sequence:
5'-CCGG-GCTTCTGACTACATCCCAGAT-CTCGAG-ATCTGGGATGTAGTCAGAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCTGACTACATCCCAGATCTCGAGATCTGGGATGTAGTCAGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCTGACTACATCCCAGATCTCGAGATCTGGGATGTAGTCAGAAGC-3'

Other clones with same target sequence:

  1. TRCN0000332217