Construct: shRNA TRCN0000076919

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013829.1-240s1c1
DNA Barcode:
GCCTCTTCAAAGTAGATGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plcb4 (18798)
Gene Description:
phospholipase C, beta 4
Transcript:
RefSeq NM_013829.1 (NON-CURRENT)
Match location:
Position 240 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000076919)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCTTCAAAGTAGATGAAT
Hairpin Sequence:
5'-CCGG-GCCTCTTCAAAGTAGATGAAT-CTCGAG-ATTCATCTACTTTGAAGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCTTCAAAGTAGATGAATCTCGAGATTCATCTACTTTGAAGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCTTCAAAGTAGATGAATCTCGAGATTCATCTACTTTGAAGAGGC-3'

Other clones with same target sequence:

(none)