Construct: shRNA TRCN0000076957

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_127051.5-2401s1c1
DNA Barcode:
GCAAATGGAATAAGCCATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sos2 (20663)
Gene Description:
son of sevenless homolog 2 (Drosophila)
Transcript:
RefSeq XM_127051.5 (NON-CURRENT)
Match location:
Position 2401 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000076957)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAATGGAATAAGCCATAAT
Hairpin Sequence:
5'-CCGG-GCAAATGGAATAAGCCATAAT-CTCGAG-ATTATGGCTTATTCCATTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAATGGAATAAGCCATAATCTCGAGATTATGGCTTATTCCATTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAATGGAATAAGCCATAATCTCGAGATTATGGCTTATTCCATTTGC-3'

Other clones with same target sequence:

  1. TRCN0000332793