Construct: shRNA TRCN0000077010

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011338.1-457s1c1
DNA Barcode:
CGGAGAGTTCAGAGATGCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccl9 (20308)
Gene Description:
chemokine (C-C motif) ligand 9
Transcript:
RefSeq NM_011338.1 (NON-CURRENT)
Match location:
Position 457 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000077010)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAGAGTTCAGAGATGCATT
Hairpin Sequence:
5'-CCGG-CGGAGAGTTCAGAGATGCATT-CTCGAG-AATGCATCTCTGAACTCTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAGAGTTCAGAGATGCATTCTCGAGAATGCATCTCTGAACTCTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAGAGTTCAGAGATGCATTCTCGAGAATGCATCTCTGAACTCTCCG-3'

Other clones with same target sequence:

(none)