Construct: shRNA TRCN0000077045

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009018.1-449s1c1
DNA Barcode:
CCCACATTTACAAGTCACCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Raet1c (19370)
Gene Description:
retinoic acid early transcript gamma
Transcript:
RefSeq NM_009018.1 (CURRENT)
Match location:
Position 449 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000077045)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACATTTACAAGTCACCAT
Hairpin Sequence:
5'-CCGG-CCCACATTTACAAGTCACCAT-CTCGAG-ATGGTGACTTGTAAATGTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACATTTACAAGTCACCATCTCGAGATGGTGACTTGTAAATGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACATTTACAAGTCACCATCTCGAGATGGTGACTTGTAAATGTGGG-3'

Other clones with same target sequence:

(none)