Construct: shRNA TRCN0000077092

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028636.1-2479s1c1
DNA Barcode:
GCTACCTATGAGATCCAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Man2c1 (73744)
Gene Description:
mannosidase, alpha, class 2C, member 1
Transcript:
RefSeq NM_028636.1 (NON-CURRENT)
Match location:
Position 2479 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000077092)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACCTATGAGATCCAGTTT
Hairpin Sequence:
5'-CCGG-GCTACCTATGAGATCCAGTTT-CTCGAG-AAACTGGATCTCATAGGTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACCTATGAGATCCAGTTTCTCGAGAAACTGGATCTCATAGGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACCTATGAGATCCAGTTTCTCGAGAAACTGGATCTCATAGGTAGC-3'

Other clones with same target sequence:

  1. TRCN0000334214